Please use this identifier to cite or link to this item: http://hdl.handle.net/11054/1949
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dc.contributorHope, C.en_US
dc.contributorHuynh, D.en_US
dc.contributorWong, Y.en_US
dc.contributorOakey, H.en_US
dc.contributorPerkins, G.en_US
dc.contributorNguyen, T.en_US
dc.contributorBinkowski, S.en_US
dc.contributorBui, M.en_US
dc.contributorChoo, A.en_US
dc.contributorGibson, E.en_US
dc.contributorHuang, D.en_US
dc.contributorKim, K.en_US
dc.contributorNgui, K.en_US
dc.contributorRawlinson, W.en_US
dc.contributorSadlon, T.en_US
dc.contributorCouper, J.en_US
dc.contributorPenno, M.en_US
dc.contributorBarry, S.en_US
dc.date.accessioned2022-06-01T23:37:11Z-
dc.date.available2022-06-01T23:37:11Z-
dc.date.issued2021-
dc.identifier.govdoc01875en_US
dc.identifier.urihttp://hdl.handle.net/11054/1949-
dc.descriptionIncludes data from BHSen_US
dc.description.abstractBackground: Rural/remote blood collection can cause delays in processing, reducing PBMC number, viability, cell composition and function. To mitigate these impacts, blood was stored at 4 °C prior to processing. Viable cell number, viability, immune phenotype, and Interferon-γ (IFN-γ) release were measured. Furthermore, the lowest protective volume of cryopreservation media and cell concentration was investigated. Methods: Blood from 10 individuals was stored for up to 10 days. Flow cytometry and IFN-γ ELISPOT were used to measure immune phenotype and function on thawed PBMC. Additionally, PBMC were cryopreserved in volumes ranging from 500 µL to 25 µL and concentration from 10 × 106 cells/mL to 1.67 × 106 cells/mL. Results: PBMC viability and viable cell number significantly reduced over time compared with samples processed immediately, except when stored for 24 h at RT. Monocytes and NK cells significantly reduced over time regardless of storage temperature. Samples with >24 h of RT storage had an increased proportion in Low-Density Neutrophils and T cells compared with samples stored at 4 °C. IFN-γ release was reduced after 24 h of storage, however not in samples stored at 4 °C for >24 h. The lowest protective volume identified was 150 µL with the lowest density of 6.67 × 106 cells/mL. Conclusion: A sample delay of 24 h at RT does not impact the viability and total viable cell numbers. When long-term delays exist (>4 d) total viable cell number and cell viability losses are reduced in samples stored at 4 °C. Immune phenotype and function are slightly altered after 24 h of storage, further impacts of storage are reduced in samples stored at 4 °C.en_US
dc.description.provenanceSubmitted by Gemma Siemensma (gemmas@bhs.org.au) on 2022-05-05T01:00:44Z No. of bitstreams: 0en
dc.description.provenanceApproved for entry into archive by Gemma Siemensma (gemmas@bhs.org.au) on 2022-06-01T23:37:11Z (GMT) No. of bitstreams: 0en
dc.description.provenanceMade available in DSpace on 2022-06-01T23:37:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021en
dc.titleOptimization of blood handling and peripheral blood mononuclear cell cryopreservation of low cell number samples.en_US
dc.typeJournal Articleen_US
dc.type.specifiedArticleen_US
dc.contributor.corpauthorENDIA Study Groupen_US
dc.bibliographicCitation.titleInternational Journal of Molecular Sciencesen_US
dc.bibliographicCitation.volume22en_US
dc.bibliographicCitation.issue17en_US
dc.bibliographicCitation.stpage9129en_US
dc.subject.healththesaurusPBMCen_US
dc.subject.healththesaurusBLOOD HANDLINGen_US
dc.subject.healththesaurusCELL CONCENTRATIONen_US
dc.subject.healththesaurusCRYOPRESERVATIONen_US
dc.subject.healththesaurusDELAY IN PROCESSINGen_US
dc.identifier.doihttps://doi.org/10.3390/ijms22179129en_US
Appears in Collections:Research Output

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