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http://hdl.handle.net/11054/1949
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DC Field | Value | Language |
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dc.contributor | Hope, C. | en_US |
dc.contributor | Huynh, D. | en_US |
dc.contributor | Wong, Y. | en_US |
dc.contributor | Oakey, H. | en_US |
dc.contributor | Perkins, G. | en_US |
dc.contributor | Nguyen, T. | en_US |
dc.contributor | Binkowski, S. | en_US |
dc.contributor | Bui, M. | en_US |
dc.contributor | Choo, A. | en_US |
dc.contributor | Gibson, E. | en_US |
dc.contributor | Huang, D. | en_US |
dc.contributor | Kim, K. | en_US |
dc.contributor | Ngui, K. | en_US |
dc.contributor | Rawlinson, W. | en_US |
dc.contributor | Sadlon, T. | en_US |
dc.contributor | Couper, J. | en_US |
dc.contributor | Penno, M. | en_US |
dc.contributor | Barry, S. | en_US |
dc.date.accessioned | 2022-06-01T23:37:11Z | - |
dc.date.available | 2022-06-01T23:37:11Z | - |
dc.date.issued | 2021 | - |
dc.identifier.govdoc | 01875 | en_US |
dc.identifier.uri | http://hdl.handle.net/11054/1949 | - |
dc.description | Includes data from BHS | en_US |
dc.description.abstract | Background: Rural/remote blood collection can cause delays in processing, reducing PBMC number, viability, cell composition and function. To mitigate these impacts, blood was stored at 4 °C prior to processing. Viable cell number, viability, immune phenotype, and Interferon-γ (IFN-γ) release were measured. Furthermore, the lowest protective volume of cryopreservation media and cell concentration was investigated. Methods: Blood from 10 individuals was stored for up to 10 days. Flow cytometry and IFN-γ ELISPOT were used to measure immune phenotype and function on thawed PBMC. Additionally, PBMC were cryopreserved in volumes ranging from 500 µL to 25 µL and concentration from 10 × 106 cells/mL to 1.67 × 106 cells/mL. Results: PBMC viability and viable cell number significantly reduced over time compared with samples processed immediately, except when stored for 24 h at RT. Monocytes and NK cells significantly reduced over time regardless of storage temperature. Samples with >24 h of RT storage had an increased proportion in Low-Density Neutrophils and T cells compared with samples stored at 4 °C. IFN-γ release was reduced after 24 h of storage, however not in samples stored at 4 °C for >24 h. The lowest protective volume identified was 150 µL with the lowest density of 6.67 × 106 cells/mL. Conclusion: A sample delay of 24 h at RT does not impact the viability and total viable cell numbers. When long-term delays exist (>4 d) total viable cell number and cell viability losses are reduced in samples stored at 4 °C. Immune phenotype and function are slightly altered after 24 h of storage, further impacts of storage are reduced in samples stored at 4 °C. | en_US |
dc.description.provenance | Submitted by Gemma Siemensma (gemmas@bhs.org.au) on 2022-05-05T01:00:44Z No. of bitstreams: 0 | en |
dc.description.provenance | Approved for entry into archive by Gemma Siemensma (gemmas@bhs.org.au) on 2022-06-01T23:37:11Z (GMT) No. of bitstreams: 0 | en |
dc.description.provenance | Made available in DSpace on 2022-06-01T23:37:11Z (GMT). No. of bitstreams: 0 Previous issue date: 2021 | en |
dc.title | Optimization of blood handling and peripheral blood mononuclear cell cryopreservation of low cell number samples. | en_US |
dc.type | Journal Article | en_US |
dc.type.specified | Article | en_US |
dc.contributor.corpauthor | ENDIA Study Group | en_US |
dc.bibliographicCitation.title | International Journal of Molecular Sciences | en_US |
dc.bibliographicCitation.volume | 22 | en_US |
dc.bibliographicCitation.issue | 17 | en_US |
dc.bibliographicCitation.stpage | 9129 | en_US |
dc.subject.healththesaurus | PBMC | en_US |
dc.subject.healththesaurus | BLOOD HANDLING | en_US |
dc.subject.healththesaurus | CELL CONCENTRATION | en_US |
dc.subject.healththesaurus | CRYOPRESERVATION | en_US |
dc.subject.healththesaurus | DELAY IN PROCESSING | en_US |
dc.identifier.doi | https://doi.org/10.3390/ijms22179129 | en_US |
Appears in Collections: | Research Output |
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